Isolated Nuclear Membranes

T h e only m e t h o d so far k n o w n for isolat ing par t s of the nuc lea r enve lope is the t echn ique of Cal lan a n d T o m l i n (3), i.e. dissect ing the envelopes of manual lyi so la ted ind iv idua l nuclei . This procedu re has been used successfully by m a n y workers (6, 8, 13, 14, s u m m a r y in reference 7). I ts g rea t d i sadvan tage is tha t it m u s t be res t r ic ted necessarily to the few mater ia l s w h i c h possess ex t remely large nuclei , e.g. the g ian t nuclei of a m p h i b i a n oocytes. To supply a founda t ion for fu r the r invest igat ions on the chemis t ry and s t ruc ture of the nuc lea r envelope , a m e t h o d has been worked ou t w h i c h allows the isolat ion of nuc lea r enve lope m e m b r a n e s in genera l , f rom an ima l tissue as well as f rom p l a n t tissue.